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The hair growth promotion effect of oral PMR was most probably mediated by the expression of FGF-7, while topical PMRP promoted hair growth by the stimulation of SHH expression.

Therefore, searching for effective and safe hair growth promotion and hair dying drugs in relative low price from traditional Chinese medicine or natural products has enormous social and economic benefits.

Polygonum multiflorum Radix (PMR) and Polygonum multiflorum Radix Preparata (PMRP), traditional Chinese medicine originated from Polygonum multiflorum, had long history for hair growth and hair dying in clinical applications. multiflorum was recorded as an extremely tonic medicine that could “benefit for the essence, strong your spirits, protect your beauty, black your hair, and extend your life” (Polygonum multiflorum Biography, AD 812). multiflorum extract could promote the quiescent C57BL/6J mice hair follicles to enter anagen stage earlier [3].

In another in vitro pig hair follicle cell culture model, PMR decoction could inhibit hair follicle apoptosis and retard the anagen stage from entering the catagen stage [4].